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ChromSpeed™ DA101 & DA103

ChromSpeed™ DA103 & DA101 is a member of the ChromSpeed™ family of ion exchange media, especially designed for large molecules including monoclonal antibodies (MAbs) at process scale. ChromSpeed™ is composed of a rigid, high-flow methacrylate matrix with excellent ion exchange capacity. Particles sizes of 30 μm and 60 μm are available for such process scale providing high selectivities. This ChromSpeed™ DA is functionalized with dimethylamine, unlike any other media with diethylaminoethyl (N(C2H5)2C2H4OH), typically referred as DEAE. This results in unique purification performance.

Characteristics of ChromSpeed™ DA101 & DA103

Grade Name	ChromSpeed™ DA101	ChromSpeed™ DA103
Functional Group	-N+H(CH3)2 (dimethylamine)
Matrix	Rigid, highly cross-linked methacrylate
Water Content (%)	30μm	60μm
Pore size*	≥ ~700A
Ion exchange capacity	≥ 0.10 meq/mL-media
Static binding capacity of humanγ-globulin**	≥ 75 g/L-media
Chemical stability	Stable in all aqueous buffers, including 20% ethanol and 2% benzyl alcohol
pH working range	1 to 12
Cleaning-in-place stability	0.1-0.5N NaOH
Temperature stability	2℃-40℃
Delivery conditions	20% ethanol, 2% benzyl alcohol

*Pore size listed above is referential data measured by mercury intrusion method.
**SBC is measured with a human γ-globulin with a 2.5 g/L, 20mM citrate (pH 5.2), 25℃.

Hydraulic data

The rigid matrix of ChromSpeed™ DA101 and DA103 enables higher flow rate operation than conventional resins. There is a linear relationship between the flow velocity and the pressure drop of the packed bed even at higher flow rate, and there is no change in resin volume or resin shape.

Figure 1. Pressure drop data. Data were taken with a condition of a column 20 mm ID x 200 mm with water at a room temperature.

Separation of standard proteins

As representative purification performance, Figure 2 depicts separation of standard proteins. The uniform media of ChromSpeed™ on the last two in the figure shows in better efficiency comparing not only competing polymer media but also other media including cellulose and agarose media. The difference of the functional group of DA to most of competing products, DEAE, has resulted in also unique separation. The figure also shows that 30μm of ChromSpeed™ DA101 offers better resolution than ChromSpeed™ DA103 of 60μm due to its difference in particle sizes.

Figure 2. Standard proteins of myoglobin and trypsin inhibitor separation. Data were taken with a column: 5mm ID x 100 mm, eluent A: 50mM Tris-HCl (pH 8.5), eluent B: A + 1.0M NaCl, flow rate: 1.0 mL/min (r.t. 2 min), gradient: 0-100% B over 60 min, samples: (a) myoglobin (pl 7.5) + (b) trypsin inhibitor (pl 4.5) = 250 / 50ug / 25uL.

Effect of pH on ChromSpeed™ DA (dimethylamine) series

Due to a difference of the functional group, compared with what's available on market, the eluting pH behavior would change as shown in Figure 3 below. This is also one of the unique characteristics of Chromspeed™ DA series.

Figure 3. Effect of pH on ChromSpeed™ DA series.

Ordering information

Product Name / Unit			Product Number
ChromSpeed™ DA101	10000 500 100 25	mL	6-401-03 6-401-02 6-401-01 6-401-00
ChromSpeed™ DA103	10000 500 100 25	mL	6-403-03 6-403-02 6-403-01 6-403-00

Related Data Sheets: Bioseparation Screening Columns : No. 03-01-C-0101

Available Documents:
-Packing procedure for ChromSpeed™ 101 series
-Packing procedure for ChromSpeed™ 103 series
-Use and care instructions for ChromSpeed™ 101 series
-Use and care instructions for ChromSpeed™ 103 series

*Regulatory Support Files (RSF) are available upon request.

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