Commercial purifications in large scale begin at a small scale in methods development. In some cases, the developers and researchers wish to use media to pack their initial small columns. This case allows one to experience how the media can be handled physically and chemically, and one can utilize the knowledge later on during the scale-up periods. In other cases, the due to the speed and convenience of having a pre-packed column for media evaluation is necessary. These screening columns, available in sizes listed in Table 1, are suitable for evaluating different types of MabSpeed™*, shown in Table 2, and ChromSpeed™, shown in Table 3 for developing the purification conditions of biological target molecules such as proteins or nucleic acids. The screening columns are available in two column volumes of 1mL and 5mL.

A ChromSpeed™ screening column is available in two different particle sizes and four different functional groups. A MabSpeed™ screening column is available in two different particle sizes. Optimal selection of a particular media could involve screening of several media.

ChromSpeed™ ion exchange media separates molecules based on the ionic interaction of the molecules with the charged support. The net surface charge of proteins is dependent on the pH and ionic strength of the mobile phases. The development of optimal chromatography conditions requires knowledge of both the protein’s pI and the pKa of the ion exchange media. In bio-purification or purifications of biologics, ChromSpeed™ can be used either in “bind/ elute mode” and/ or in “flow-through mode.” Ion exchange media should be selected according to the properties of the feedstock and the objective of the process steps. ChromSpeed™ media are designed for such a large molecules with large pore sizes, mainly dominated in the range of 500 to 1000 A, offering large binding capacity. Some of typical applications is shown in Figure 1 and 2 as well as Figure 3.

MabSpeed™ rP111 was used for the purification of a monoclonal antibody from CHO cell culture supernatant with a concentration of 0.5 mg/mL at 3 minutes residence time in a 15 cm bed height column. Consecutively, the processed fluid was purified with ChromSpeed™ S101 and ChromSpeed™ Q101, as a flow of Figure 4 shows, and the intermediate and final products were analyzed with regard to yield, dimers, aggregates, Protein A carry over, and Host Cell Proteins (HCP) as shown in the following Table 4.