충인과학

Column Selection Guide for the Analysis and Purification of
Proteins and Peptides by Reversed-Phase HPLC

Particle Size	Phase	Polymer 259VHP	Butyl 214TP	Octyl 208TP	ODS 218TP	ODS 238TP	Diphenyl 219TP	SP ODS 201 SP
	Application	Proteins and peptides requiring extreme conditions for dissolution and separation (acid, alkli, heat)	Proteins, large peptides, hydrophobic polypeptides and cyanogen bromide digests	Peptides and enzymatic digests	Enzymatic digests mapping, analysis and puriflcation of synthetic and natural peptides (polymeric bonding)	Enzymatic digests mapping, analysis and puriflcation of synthetic and natural peptides (monomeric bonding	Unique separation needs that benefit from the aromatic reverse phase, hydrophobic proteins	Small peptides (>10aa), particularly basic or hydrophilic peptides
	Column Size
3 micron	Fast Analytical 4.6 x 50 mm 4.6 x 100 mm		214TP3405 214TP3410	208TP3405 208TP3410	218TP3405 218TP3410	238TP3405 238TP3410	219TP3405 219TP3410
5 micron	Microbore 1.0 x 50 mm 1.0 x 150 mm 1.0 x 250 mm		214TP5105 214TP5115 214TP51	208TP5105 208TP5115 208TP51	218TP5105 218TP5115 218TP51	238TP5105 238TP5115 238TP51	219TP5105 219TP5115 219TP51	F 201TP5115 201TP51
	Narrowbore 2.1 x 50 mm 2.1 x 150 mm 2.1 x 250 mm Recommended Guard Kit * Recommended Guard Cartridges **	F 259VHP5205 259VHP5215 259VHP52	F 214TP5205 214TP5215 214TP52 214GK52 214GD52	F 208TP5205 208TP5215 208TP52 208GK52 208GD52	F 218TP5205 218TP5215 218TP52 218GK52 218GD52	238TP5205 238TP5215 238TP52 238GK52 238GD52	219TP5205 219TP5215 219TP52 219GK52 219GD52	F 201SP5215 201SP52 201GK52SP 201GD52SP
	Analytical/Semipreparative
	3.2 x150 mm 3.2 x 250 mm 4.6 x 50 mm 4.6 x 150 mm 4.6 x 250 mm 10 x 250 mm Recommended Guard Kit * Recommended Guard Cartridges **	F F 259VHP5405 259VHP5415 259VHP54	214TP5315 214TP53 214TP5405 214TP5415 214TP54 214TP510 214GK54 214GD54	208TP5315 208TP53 208TP5405 208TP5415 208TP54 208TP510 208GK54 208GD54	218TP5315 218TP53 218TP5405 218TP5415 218TP54 218TP510 218GK54 218GD54	238TP5315 238T53 238TP5405 238TP5415 238TP54 238TP510 238GK54 228GD54	219TP5315 219TP53 219TP5405 219TP5415 219TP54 219TP510 219GK54 219GD54	F F F 201SP5415 201SP54 201SP510 201GK54SP 201GD54SP
8 micron	10 x 250 mm 22 x 250 mm	259VHP810 259VHP822
10 micron	4.6 x 150 mm 4.6x 250 mm 10 x 250 mm Recommended Guard Kit * Recommended Guard Cartridges **		214TP10415 214TP104 214TP1010 214GK104 214GD104	208TP10415 208TP104 208TP1010 208GK104 208GD104	218TP10415 218TP104 218TP1010 218GK104 218GD104	238TP10415 238TP104 238TP1010 238GK104 238GD104	219TP10415 219TP104 219TP1010 219GK104 219GD104	201SP10415 201SP104 201SP1010 201GK104SP 201GD104SP
10 micron	Preparative /Process 22 x 250 mm		214TP1022	208TP1022	218TP1022	238TP1022	219TP1022	201SP1022
10-15 micron	22 x 250 mm 50 x 250 mm 100 x 250mm	259VHP1522 259VHP1550 259VHP15100	214TP101522 214TP101550 214TP15100	208TP101522 208TP101550 208TP1015100	218TP101522 218TP101550 218TP1015100	238TP101522 238TP101550 238TP1015100	219TP101522 219TP101550 219TP1015100	201SP1522> 201SP1550 201SP15100
15-20 micron	22 x 250 mm 50 x 250 mm 100 x 250mm		214TP152022 214TP152050 214TP1520100	208TP152022 208TP152050 208TP1520100	218TP152022 218TP152050 218TP1520100	238TP152022 238TP152050 238TP1520100	219TP152022 219TP152050 219TP1520100
Guards for 22mm i.d. preparative column only	Recommended Guard Kit * Recommended Guard Cartridges **		214FSK1210 214GCC1210	208FSK1210 208GCC1210	218FSK1210 218GCC1210	238FSK1210 238GCC1210	219FSK1210 219GCC1210	201FSK1210SP 201GCC1210SP
Preparativeguardcolumn	25 x 30 mm 15-20 micron particle size		214TP15202503	208TP15202503	218TP15202503	238TP15202503	219TP15202503	201SP152503§
	Bulk Material
10-5 micron		259VHPB15	214TPB1015	208TPB1015	218TPB1015	238TPB1015	219TPB1015	201SPB15§
15-20 micron			214TPB1520	208TPB1520	218TPB1520	238TPB1520
20-30 micron			214TPB2030	208TPB2030	218TPB2030	238TPB2030	219TPB2030

For laboratory use bulk material is available in quantities of 100 and 500 grams. For process HPLC needs please request a quotation for quantities of 1-100 kg or larger.
* Includes a holder and one cartridge ** Tow cartridges per package § 15 µm particle size